A simple lipase assay using trichloroacetic acid active glycerol hydrolyzed by the enzyme from residual 3H-labeled glyceryl trioleate. MICHAEL
نویسندگان
چکیده
Medicine, U C L A Center for the Health Sciences, Los Angeles, California 90024 trioleate (266 mCi/mmole), Amersham/Searle, Arlington Heights, Ill. ; bovine albumin, fatty acid-poor, Miles Research Products, Kankakee, 111. ; Triton X-100, Rohm and Haas, Philadelphia, Pa. ; and egg lecithin, Schwarz/Mann, Orangeburg, N.Y. The egg lecithin was purified by a water wash of a chloroform-methanol 2 : 1 (v/v) extract (6). All other chemicals used were reagent grade. Pasteur pipettes, 14.5 cm long, were plugged at the constriction with a small amount of glass wool (approximately 40 mg). The assay was carried out in a disposable
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